标题: Future of 军团菌 Monitoring: Strategies for Employing qPCR in a WMP.
学习目标:
我们将提供数据,概述现代的情况, 快速, microbiological technologies are currently available, 在很多情况下,这是一个更好的评估选择 军团菌 建造供水系统的风险.
内容/主题大纲:
推荐者:
迈克尔Loewenstein
报告描述:
文化方法(1).e., iso 11731) is often purported to be the “Gold Standard” in 军团菌 测试. 然而, there are many known problems associated with this method – the most often noted being its extraordinarily long time to result which can be up to two weeks or more. This lengthy time to result puts the water system operator in a constantly 无功 位置, 强迫系统“冒着风险”使用, or even taken out of service for weeks or months at a time, 每当一个重要的 军团菌 观察到恢复.
We reported to AWT at the 2021 Annual Convention on results from Phase I of a study we conducted evaluating three distinct modern, PCR/qPCR-based technologies that employ “live/dead” differentiation chemistries. Phase I of this study compared these methods to one another as well as to the cultural method for lab-inoculated water samples. All three 快速 methods showed equivalent or better qualitative detection efficiency in one day, compared to the 7-14 days required for the cultural method. We are currently in Phase II of the study which is comparing the most promising of the three 快速 methods to the cultural method, utilizing samples gathered in various end-use field applications. Thus far, we are observing an alarming trend of detection of 军团菌 仕达屋优先计划. via qPCR in the same samples for which detection was missed by culture – yielding (at time of publication) a hugely disturbing 63% false negative rate with the cultural method. This is consistent with data we collected in Phase I which showed that the cultural method had poor qualitative detection efficiency at low levels of 军团菌 污染.
The good news is that these data demonstrate that there are now multiple, accurate, 快速 军团菌 测试文化方法的替代方法, and technical improvements in recent years make them an excellent choice for routine 军团菌 监控. 从实际的角度来看, it is easy to grasp how a faster time to result would mean that water treaters could be more agile in their approach to managing the health of their water systems. 然而,这些新的数据表明,快速的方法, 在很多情况下, are the optimal choice for obtaining the most accurate assessment of Legionella 设施供水系统的风险.
的敏感性, 精度, and faster time to result of qPCR puts the water management team in a far more 积极主动的 位置 to efficiently detect 军团菌, assess the situation, and take appropriate steps to mitigate the risk.
主持人生物:
迈克尔Loewenstein
迈克尔Loewenstein is the current Vice President of Scientific Consulting at Q Labs LLC in Cincinnati, OH. 在这个职位上,Mr. Loewenstein serves as the technical lead of much of Q Labs’ business including Water Treatment, 并领导着Q实验室技术进步小组. Mr. Loewenstein获得了B.S. 在微生物学, with a research focus in Molecular Genetics and Cellular Physiology from The Ohio State University. After graduation, Michael obtained a 位置 in Corporate R&D宝洁公司的微生物学 & Gamble where he focused on the development and validation of 快速/alternative microbial test methods, developed and launched preservative systems across a broad range of P&G产品, and has developed particular expertise and passion for microbiological control of manufacturing. 他已经就这个问题发表了几篇文章, 包括干净的设计, and 监控 programs of industrial and purified water systems. Michael currently serves in a volunteer capacity for several professional associations, including the AOAC Microbial Contaminants Expert Review Panel, and is a founding member of Manufacturing Hygiene Task Force of the Microbiology Committee for the Personal Care Products Council (PCPC). Michael is also looking forward to becoming more involved with the Cooling Water subcommittee of AWT!